Tuesday, June 18, 2019

Difficulties in Determining Concentration

The new curcumin reaction taking place in the lab could possibly be very useful.  However, HPLC has confirmed that the reaction produces a number of side products, which is not uncommon or unexpected.  This means it will be impossible to determine the extinction coefficient of the new compound.  The extinction coefficient is necessary to determine the concentration of this new compound in solution.  UV spectra will have to serve only as a means of determining the maximum wavelength of the compound.  Once an LCMS spectra is run, it will be easier to separate the major product of the reaction from the other side products.


-Alexandra Griffith
6/18/19

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